Research Interests
My main area of interest is glutamate homeostasis in the central
nervous system and I am particularly interested in regulation
of the glutamate transporters (also known as Excitatory Amino Acid
Transporters) such as EAAT1, EAAT2 and EAAT5. EAATs are
typically present as alternately spliced forms, the splicing
potentially conferring differing properties either alone, or when
present as combinations of variant forms. I have recently cloned
new EAAT splice variants from tissues such as brain and retina and
have initiated studies to investigate their expression and
regulation in normal and disease states.
Other areas of interest include:
(1) Cloning and characterizing the 5'-flanking (promoter)
regions of EAATs in order to gain insights into their regulation at
the transcriptional level.
(2) Identification of novel proteins that interact with EAATs
and the role of protein-protein interactions responsible for
modulation of EAAT abundance and activity.
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Key publications
Lee A, Anderson AR, Barnett AC, Chan A, Pow DV.
(2011) Asian J Androl. 13(2):254-65.
Lee A, Lingwood B, Bjorkman ST, Miller SM,
Poronnik P, Barnett NL, Colditz PB, Pow DV (2010) J Chem
Neuroanat 39(3):211-20
Sullivan SM,
Lee A,
Björkman ST,
Miller SM,
Sullivan RK,
Poronnik P,
Colditz PB,
Pow DV. (2007) J Biol Chem 282(40):29414-23
Lee A,
Rayfield A,
Hryciw DH,
Ma TA,
Wang D,
Pow D,
Broer S,
Yun C,
Poronnik P. (2007) Glia 55(2):119-29
REVIEW
Lee A, Pow DV. (2010) Int J Biochem Cell
Biol. 42(12):1901-6.
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Contact details and email
Dr Aven Lee
UQCCR, Building 71/918, RBWH
Herston, Brisbane, QLD, 4029
AUSTRALIA
Ph: +61 7 334 66010
Fax: +61 7 334 65594
Email: aven.lee@uq.edu.au
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Technique Expertise
Isolation/purification of total RNA and Poly(A+) RNA
from tissues
DNA cloning
cDNA library construction and screening
RT-PCR, semi-quantitative RT-PCR
RACE, Genome walking by PCR
Site directed mutagenesis using PCR
Northern blotting
Southern blotting
Western blotting
Recombinant protein expression and purification
GST-pulldown
Co-immunoprecipitation
Adenovirus
Lentivirus
Tissue culture and transfection
Xenopus laevis oocytes and Transport assays
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Collaborations
David Pow, UQCCR
Phillip Poronnik, RMIT, Victoria
Rebecca Lim, University of Newcastle, NSW
David Cook, Washington University, Seattle
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